Proteomics Facility

The Proteomics Facility provides a variety of proteomic services including protein molecular weight determination, identification and analysis.  This facility will enable FAMU Biomedical Researchers to answer pressing biological questions about the functions and roles of proteins in diseases impacting the underserved and minority populations.  With sample preparation, separations, mass spectrometry, and informatics, we will be able to provide information that drives knowledge about disease pathology and target based drug discovery will be realized.

Equipment:

  • ABSciex 4800 MALDI TOF-TOF Mass Spectrometer
  • Protean IEF System
  • TM-Sprayer
  • Bio-Rad VersaDoc 4000 Imager
  • Phospho Imager
  • Creiterion Dodeca Cell and Criterion Cell

Specific Services:

  • Protein separation using either 2D-Gel electrophoresis
  • Gel imaging using Bio-Rad VersaDoc Imager
  • Archiving and retrieval of proteomics data for protein informatics analysis
  • Sample preparation
  • Provide consultation on experimental design
  • Provide individual/group proteomics training
  • Digest samples analyzed using ABSciex 4800 MALDI-TOF-TOF
  • Gel spot cutting, trypsin digestion and peptide extraction

Staff: The Proteomics Facility will be managed and operated by Dr. Equar Taka (equar.taka@famu.edu) and Dr. Tracy Womble (tracy.womble@famu.edu) under the supervision of Dr. Selina Darling-Reed (Selina.darling@famu.edu)

Fee Structure: Please contact either of the facility managers, Dr. Tracy Womble (850-561-2466) or Dr. Equar Taka (850-599-3127) regarding fees. At present, the facility would not charge any fee to FAMU RCMI or non-RCMI faculty members for the services provided (this is subject to change). The facility staff, however strongly encourage the students and faculty to gain valuable experience in using the software for their perspective research projects. The facility staff is responsible for training, trouble shooting, obtaining licenses and implementation of the requested software. If a particular staff of the facility is involved in more than 50 % of the computational work in a student/PI s research, he is expected to be included as an author in the student/PI’s publication/patent or conference abstract/poster. If the contribution of the staff is less than 50% he and the facility would be expected to be acknowledged in the abstracts/posters/publications as deemed appropriate for their services.